CSpace  > 2018期刊论文
Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing
Veerapandian, Veeramohan; Ackermann, Jan Ole; Srivastava, Yogesh; Malik, Vikas; Weng, Mingxi; Yang, Xiaoxiao; Jauch, Ralf
2019-08-14
Source PublicationSTEM CELL REPORTS
Volume11Issue:2Pages:593-606
AbstractDirected biomolecular evolution is widely used to tailor and enhance enzymes, fluorescent proteins, and antibodies but has hitherto not been applied in the reprogramming of mammalian cells. Here, we describe a method termed directed evolution of reprogramming factors by cell selection and sequencing (DERBY-seq) to identify artificially enhanced and evolved reprogramming transcription factors. DERBY-seq entails pooled screens with libraries of positionally randomised genes, cell selection based on phenotypic readouts, and genotyping by amplicon sequencing for candidate identification. We benchmark this approach using pluripotency reprogramming with libraries based on the reprogramming factor SOX2 and the reprogramming incompetent endodermal factor SOX17. We identified several SOX2 variants outperforming the wild-type protein in three- and four-factor cocktails. The most effective variants were discovered from the SOX17 library, demonstrating that this factor can be converted into a highly potent inducer of pluripotency with a range of diverse modifications. We propose DERBY-seq as a broad-based approach to discover reprogramming factors for any donor/target cell combination applicable to direct lineage reprogramming in vitro and in vivo.
Indexed BySCIE
Document Type期刊论文
Identifierhttp://ir.foo.ac.cn/handle/2SETSVCV/1470
Collection2018期刊论文
Recommended Citation
GB/T 7714
Veerapandian, Veeramohan,Ackermann, Jan Ole,Srivastava, Yogesh,et al. Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing[J]. STEM CELL REPORTS,2019,11(2):593-606.
APA Veerapandian, Veeramohan.,Ackermann, Jan Ole.,Srivastava, Yogesh.,Malik, Vikas.,Weng, Mingxi.,...&Jauch, Ralf.(2019).Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing.STEM CELL REPORTS,11(2),593-606.
MLA Veerapandian, Veeramohan,et al."Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing".STEM CELL REPORTS 11.2(2019):593-606.
Files in This Item: Download All
File Name/Size DocType Version Access License
2018067DDP(Ralf)-Dir(4978KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Veerapandian, Veeramohan]'s Articles
[Ackermann, Jan Ole]'s Articles
[Srivastava, Yogesh]'s Articles
Baidu academic
Similar articles in Baidu academic
[Veerapandian, Veeramohan]'s Articles
[Ackermann, Jan Ole]'s Articles
[Srivastava, Yogesh]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Veerapandian, Veeramohan]'s Articles
[Ackermann, Jan Ole]'s Articles
[Srivastava, Yogesh]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2018067DDP(Ralf)-Directed evolution of reprogramming factors by cell selection and sequencing.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.