CSpace  > 2018期刊论文
Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3 '-UTRs of meiotic mRNAs
Wu, Yaoyao; Xu, Kaibiao; Qi, Huayu
2018-10-01
Source PublicationBIOLOGY OF REPRODUCTION
Volume99Issue:4Pages:773-788
AbstractTranslational regulation plays a central role during post-meiotic development of male germ cells. Previous studies suggested that P-element induced wimpy testis like 1 (PIWIL1), a PIWI-interacting RNA (piRNA) binding protein that is critical for sperm development, participates in the maintenance and translational regulation of post-meiotic mRNAs in haploid spermatids. However, how PIWIL1 regulates protein translation remains largely unclear. Using biochemical assays, we show here that PIWIL1 utilizes different domains to interact with post-meiotic mRNAs and Poly-A binding protein cytoplasmic 1 (PABPC1), a general protein translation regulator. PIWIL1 binds 3'-untranslated regions (3'-UTRs) of several spermiogenic mRNAs via its N-terminal domain, whereas its interactions with PABPC1 are mediated through its N- and C-terminal domains in an RNA-dependent manner. Using a heterologous cell system, we analyzed its effects on protein translation via luciferase reporter assay and sucrose gradient sedimentation. It was found that PIWIL1 augments protein translation with PABPC1 in the presence of 3'-UTRs of post-meiotic mRNAs. While both the N-terminal RNA recognition motif (RRM) domain and the central linker region of PABPC1 stimulate translation, only the PIWI Argonaute and Zwille (PAZ) domain of PIWIL1 positively affects translation of reporter mRNAs. Interestingly, the PAZ domain was found absent from polysomal fractions, in contrast to the N-and C-terminal domains of PIWIL1. Taken together, the results suggest that PIWIL1 interacts with various partners using different domains and participates in translational regulation partly through 3'-UTRs. It will be of interest to further explore how PIWIL1 elicit its versatile functions, including translational regulation of post-meiotic mRNAs through intrinsic structural changes and extrinsic signals during mouse spermiogenesis under more physiological settings. Summary Sentence PIWIL1 and PABPC1 synergistically stimulate protein translation of in the presence of 3'-UTRs from mouse post-meiotic mRNAs.
KeywordPIWIL1 PABPC1 protein translation post-meiotic mRNAs 3 '-UTR
Indexed BySCIE
Document Type期刊论文
Identifierhttp://ir.foo.ac.cn/handle/2SETSVCV/1476
Collection2018期刊论文
Recommended Citation
GB/T 7714
Wu, Yaoyao,Xu, Kaibiao,Qi, Huayu. Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3 '-UTRs of meiotic mRNAs[J]. BIOLOGY OF REPRODUCTION,2018,99(4):773-788.
APA Wu, Yaoyao,Xu, Kaibiao,&Qi, Huayu.(2018).Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3 '-UTRs of meiotic mRNAs.BIOLOGY OF REPRODUCTION,99(4),773-788.
MLA Wu, Yaoyao,et al."Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3 '-UTRs of meiotic mRNAs".BIOLOGY OF REPRODUCTION 99.4(2018):773-788.
Files in This Item: Download All
File Name/Size DocType Version Access License
2018074戚华宇.pdf(2776KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, Yaoyao]'s Articles
[Xu, Kaibiao]'s Articles
[Qi, Huayu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Yaoyao]'s Articles
[Xu, Kaibiao]'s Articles
[Qi, Huayu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Yaoyao]'s Articles
[Xu, Kaibiao]'s Articles
[Qi, Huayu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 2018074戚华宇.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.