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人脂联素受体1和2的真核表达
张烁1; 刘瑜1; 吴东海2; 胡仁明1; 李益明1
2006-06
Source Publication复旦学报(医学版)
Volume33Issue:6Pages:845-847
Abstract

目的扩增和表达人脂联素受体1(AdipoR1)和人脂联素受体2(AdipoR2)基因。方法利用RT-PCR方法扩增AdipoR1和AdipoR2全长cDNA,将AdipoR1和AdipoR2的cDNA构建到真核表达载体pcDNA3.1,重组质粒转染HEK293细胞,通过免疫荧光技术标记HEK293细胞的AdipoR1和AdipoR2融合蛋白,共聚焦显微镜观察AdipoR1和AdipoR2在细胞中的定位。结果成功构建真核表达重组质粒pcDNA3.1-adipoR1和pcDNA3.1-adipoR2,重组质粒转染HEK293细胞后,定位于HEK293细胞膜上。结论AdipoR1和AdipoR2重组质粒的构建和在HEK293细胞中的成功表达,为进一步研究其与胰岛素抵抗的关系创造了条件。

Keyword脂联素受体1 脂联素受体2 融合蛋白 胰岛素抵抗
MOST Discipline Catalogue理学::生物学 ; 医学::基础医学(可授医学、理学学位)
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Indexed ByCSCD
Language中文
CSCD IDCSCD:2609363
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Document Type期刊论文
Identifierhttp://ir.foo.ac.cn/handle/2SETSVCV/899
Collection中国科学院广州生物医药与健康研究院
Affiliation1.复旦大学附属华山医院内分泌科
2.中国科学院广州生物医药与健康研究院
Recommended Citation
GB/T 7714
张烁,刘瑜,吴东海,等. 人脂联素受体1和2的真核表达[J]. 复旦学报(医学版),2006,33(6):845-847.
APA 张烁,刘瑜,吴东海,胡仁明,&李益明.(2006).人脂联素受体1和2的真核表达.复旦学报(医学版),33(6),845-847.
MLA 张烁,et al."人脂联素受体1和2的真核表达".复旦学报(医学版) 33.6(2006):845-847.
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